Quantitative analysis of joint molecule production was accomplished by scanning the photographic negative or autoradiogram using a zeineh soft laser densitometer interfaced to a hewlettpackard 3390a. Cleavage of human dna with restriction enzymes that. A cloning vector is simply a dna molecule possessing. Dna cloning with plasmid vectors molecular cell biology ncbi. Agtlo and agtll are examples of this type of vector. In the case of dna, this is feasible for relatively short molecules such as the genomes of small viruses. Cloning vector characteristics and types cloning vector. Cloning vectorcharacteristics and types online biology. The essence of cell chemistry is to isolate a particular cellular component and then analyze its chemical structure and activity.
Alkaline agarose gel electrophoresis of the samples was done according to sambrook et al. These studies revealed two features that detract from the plasmids effectiveness as a cloning vector. In molecular cloning, a vector is a dna molecule used as a vehicle to artificially carry foreign. The molecule is a doublestranded circle 4,361 base pairs in length. Features of cloning vectors definition, examples, diagrams. But genomes of even the simplest cells are much too large to directly analyze in detail at the molecular level. Dna cloning with plasmid vectors molecular cell biology. Thermo scientific pbr322 is one of the most commonly used li cloning vectors. Cloning vector is used for replicating donor dna fragment within host cell. Expression of luciferase activity in cells transfected with this plasmid depends on insertion. Cloning vector is a small dna molecule capable of selfreplication inside the host cell. Cloning vector is used increasing the number of copies of a cloned dna.